Main article: Bosses
Queen Bee is summoned by destroying the Queen Bee’s larva, encased within the walls of Bee Hives located in the Underground Jungle. It can also be summoned by the use of an Abeemination anywhere in the Jungle biome. It is the only source of Bee Wax. Queen Bee is immune to knockback.
Queen Bee alternates between charging at the player and hovering above them whilst spawning bees. In Expert Mode, the rate at which it charges the player gets faster as it takes damage.
Defeating Queen Bee will allow the Witch Doctor to spawn; other than this, it doesn’t advance the game in a major sense, but it does provide some decent loot that can further help the player prepare for the boss battle against the Wall of Flesh.